Molecular logic lab-on-a-vesicle for intracellular diagnostics

Informazioni relative al progetto

LOGIC LAB

ID dell’accordo di sovvenzione: 813920

DOI

10.3030/813920

Progetto chiuso

Data della firma CE 17 Agosto 2018

Data di avvio 1 Novembre 2018

Data di completamento 31 Ottobre 2023

Finanziato da

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Costo totale

€ 3 538 684,44

Contributo UE

€ 3 538 684,44

Coordinato da

LEIBNIZ-INSTITUT FUER PHOTONISCHE TECHNOLOGIEN E.V.
Germany

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Other (6)

Awarding of doctoral degrees
Network meeting 4

Network meeting 4 is delivered

Final symposium

Final symposium is held

Network meeting 1

Network meeting 1 is delivered

Network meeting 3

Network meeting 3 is delivered

Network meeting 2

Network meeting 2 is delivered

Documents, reports (9)

Delivery of supramolecular logic gates to living endothelial cells / co-cultures

Based on results from D2.2, a protocol to deliver supramolecular logic gates to living endothelial cells / co-cultures is developed

Animal disease models

Suitable animal disease models are identified

Living 2D cell cultures and co-cultures

Living 2D cell cultures and co-cultures are available

Proof-of-principle for TTA-UC supramolecular sensing

Proof-of-principle for TTA-UC supramolecular sensing is shown

Delivery of supramolecular logic gates to a MemSense or GUV membrane model

A protocol for delivery of supramolecular logic gates to a MemSense or GUV membrane model is established

Metabolomic profile of ED pathology

A metabolomic profile of ED pathology is established

Theoretical models for the excited state levels of the sensors

Theoretical models for the excited state levels of the sensors are established

Stable and well-characterized liposomes

Stable and well-characterized liposomes are prepared to transport the logic gates in buffer and serum

Photochemical and photophysical characteristics

Photochemical and photophysical characteristics are known

Websites, patent fillings, videos etc. (1)

ESR positions are advertised

ESR positions are advertised at national and European-wide portals and the LOGIC LAB website (e.g. Euraxess)

Open Research Data Pilot (1)

Research results are published

Research results are published in peer-reviewed, open access journals and books

Pubblicazioni

Peer reviewed articles (26)

Uptake and anti-inflammatory effects of liposomal astaxanthin on endothelial cells tracked by Raman and fluorescence imaging

Autori: Radwan, Basseem Prabhakaran, Amrutha Rocchetti, Stefano Matuszyk, Ewelina Keyes, Tia E. Baranska, Malgorzata
Pubblicato in: Microchim Acta, Numero 190, 2023, ISSN 0026-3672
Editore: Springer Verlag
DOI: 10.1007/s00604-023-05888-8

EdU sensing: The Raman way of following endothelial cell proliferation in vitro and ex vivo

Autori: Basseem Radwan; Stefano Rocchetti; Ewelina Matuszyk; Magdalena Sternak; Maciej Stodulski; Robert Pawlowski; Jacek Mlynarski; Krzysztof Brzozowski; Stefan Chlopicki; Malgorzata Baranska
Pubblicato in: Biosensors and Bioelectronics, Numero 216, 2022, ISSN 0956-5663
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2022.114624

Combining nitric oxide and calcium sensing for the detection of endothelial dysfunction

Autori: Andreeva, Valeriia D. Ehlers, Haley R. C, Aswin Krishna Presselt, Martin J. Van Den Broek, Lenie Bonnet, Sylvestre
Pubblicato in: Communications Chemistry, 2023, ISSN 2399-3669
Editore: Springer
DOI: 10.1038/s42004-023-00973-8

Radiative lifetime of a BODIPY dye as calculated by TDDFT and EOM-CCSD methods: solvent and vibronic effects

Autori: Rengel Cane E. Sia, Ruben Arturo Arellano-Reyes, Tia E. Keyes , Julien Guthmuller
Pubblicato in: Physical Chemistry Chemical Physics, Numero 23, 2021, Pagina/e 26324-26335, ISSN 1463-9084
Editore: Royal Socienty of Chemistry
DOI: 10.1039/d1cp03775g

Interaction of Miltefosine with Microcavity Supported Lipid Membrane: Biophysical Insights from Electrochemical Impedance Spectroscopy

Autori: Nirod Kumar Sarangi, Amrutha Prabhakaran, Tia E. Keyes
Pubblicato in: Electroanalysis, 2020, ISSN 1040-0397
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/elan.202060424

Excited-State Dynamics in Borylated Arylisoquinoline Complexes in Solution and <i>in cellulo</i>

Autori: Yang, T. X. Valavalkar, A. Romero-Arenas, A. Dasgupta, A. Then, P. Chettri, A. Eggeling, C. Ros, A. Pischel, U. Dietzek-Ivansic, B.
Pubblicato in: Chemistry-a European Journal, 2023, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202203468

Monitoring excited-state relaxation in a molecular marker in live cells–a case study on astaxanthin

Autori: Tingxiang Yang, Avinash Chettri, Basseem Radwan, Ewelina Matuszyk, Malgorzata Baranska, Benjamin Dietzek
Pubblicato in: Chemical Communications, 2021, ISSN 1359-7345
Editore: Royal Society of Chemistry
DOI: 10.1039/d1cc01907d

Labeled vs. Label-Free Raman Imaging of Lipids in Endothelial Cells of Various Origins

Autori: Basseem Radwan; Adriana Adamczyk; Szymon Tott; Krzysztof Czamara; Katarzyna Kaminska; Ewelina Matuszyk; Malgorzata Baranska
Pubblicato in: Molecules, Numero 25, 2020, Pagina/e 5752-5760, ISSN 1420-3049
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules25235752

Multiplex Raman imaging of organelles in endothelial cells

Autori: Ewelina Matuszyk, Adriana Adamczyk, Basseem Radwan, Anna Pieczara, Piotr Szcześniak, Jacek Mlynarski, Katarzyna Kamińska, Malgorzata Baranska
Pubblicato in: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Numero 255, 2021, Pagina/e 119658, ISSN 1386-1425
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.saa.2021.119658

Using Biological Photophysics to Map the Excited‐State Topology of Molecular Photosensitizers for Photodynamic Therapy

Autori: Avinash Chettri; Tingxiang Yang; Houston D. Cole; Ge Shi; Colin G. Cameron; Sherri A. McFarland; Benjamin Dietzek‐Ivanšić
Pubblicato in: Angewandte Chemie International Edition, Numero 62, 2023, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202301452

A BODIPY‐Based Molecular Rotor in Giant Unilamellar Vesicles: A Case Study by Polarization‐Resolved Time‐Resolved Emission and Transient Absorption Spectroscopy

Autori: Keshav Kumar Jha; Amrutha Prabhakaran; Lukas Spantzel; Rengel Cane Sia; Iván Pérez; Ruben Arturo Arellano‐Reyes; Anna Elmanova; Anindita Dasgupta; Christian Eggeling; Michael Börsch; Julien Guthmuller; Martin Presselt; Tia E. Keyes; Benjamin Dietzek‐Ivanšić
Pubblicato in: ChemPhotoChem, Numero 7, 2023, ISSN 2367-0932
Editore: John Wiley & Sons, Ltd
DOI: 10.1002/cptc.202300091

Multimodal Investigation into the Interaction of Quinacrine with Microcavity-Supported Lipid Bilayers

Autori: Nirod Kumar Sarangi Amrutha Prabhakaran Tia E. Keyes
Pubblicato in: Langmuir, Numero 38 (20), 2022, Pagina/e 6411–6424, ISSN 0743-7463
Editore: American Chemical Society
DOI: 10.1021/acs.langmuir.2c00524

Triplet Formation and Triplet-Triplet Annihilation Upconversion in Iodine Substituted Non-Orthogonal BODIPY-Perylene Dyads

Autori: Jha, K. K. Prabhakaran, A. Sia, R. C. Reyes, R. A. A. Sarangi, N. K. Yang, T. X. Kumar, K. Kupfer, S. Guthmuller, J. Keyes, T. E. Dietzek-Ivansic, B.
Pubblicato in: Chemphotochem, 2023, ISSN 2367-0932
Editore: John Wiley & Sons
DOI: 10.1002/cptc.202300073

Supramolecular Reorientation During Deposition Onto Metal Surfaces of Quasi-Two-Dimensional Langmuir Monolayers Composed of Bifunctional Amphiphilic, Twisted Perylenes

Autori: Hupfer, M. L. Meyer, R. Deckert-Gaudig, T. Ghosh, S. Skabeev, A. Peneva, K. Deckert, V. Dietzek, B. Presselt, M.
Pubblicato in: Langmuir, Numero 37, 2021, ISSN 0743-7463
Editore: American Chemical Society
DOI: 10.1021/acs.langmuir.1c01525

In Cellulo Light‐Induced Dynamics in a BODIPY‐Perylene Dyad

Autori: Tingxiang Yang; Ruben Arturo Arellano-Reyes; Rhianne C. Curley; Keshav Kumar Jha; Avinash Chettri; Tia E. Keyes; Benjamin Dietzek-Ivanšić
Pubblicato in: Chemistry – A European Journal, Numero 29, 2023, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202300224

Shorter Alkyl Chains Enhance Molecular Diffusion and Electron Transfer Kinetics between Photosensitisers and Catalysts in CO2-Reducing Photocatalytic Liposomes

Autori: David M. Klein Dr. Santiago Rodríguez-Jiménez Marlene E. Hoefnagel Dr. Andrea Pannwitz Amrutha Prabhakaran Dr. Maxime A. Siegler Prof. Tia E. Keyes Prof. Erwin Reisner Prof. Albert M. Brouwer Prof. Sylvestre Bonnet
Pubblicato in: Chemistry - A European Journnal, Numero 27, 2021, Pagina/e 17203-17212, ISSN 1521-3765
Editore: Wiley-VCH GmbH, Weinheim
DOI: 10.1002/chem.202102989

Leaflet by Leaflet Synergistic Effects of Antimicrobial Peptides on Bacterial and Mammalian Membrane Models

Autori: Arpita Roy; Nirod Kumar Sarangi; Surajit Ghosh; Amrutha Prabhakaran; Tia E. Keyes
Pubblicato in: The Journal of Physical Chemistry Letters, Numero 14, 2023, ISSN 1948-7185
Editore: American Chemical Society
DOI: 10.1021/acs.jpclett.3c00119

Toward Raman Subcellular Imaging of Endothelial Dysfunction

Autori: Adriana Adamczyk, Ewelina Matuszyk, Basseem Radwan, Stefano Rocchetti, Stefan Chlopicki, Malgorzata Baranska
Pubblicato in: Journal of Medicinal Chemistry, Numero 64/8, 2021, Pagina/e 4396-4409, ISSN 0022-2623
Editore: American Chemical Society
DOI: 10.1021/acs.jmedchem.1c00051

Dichroic Dipole Antenna Membranes from Aligned Linear BOPHY Dyes

Autori: Hupfer, M. L. Ghosh, S. Wang, Yingchun Opsomer, Tomas Dehaen, W. Presselt, M.
Pubblicato in: Advanced Materials Interfaces, 2021, ISSN 2196-7350
Editore: John Wiley & Sons
DOI: 10.1002/admi.202101490

Astaxanthin as a new Raman probe for biosensing of specific subcellular lipidic structures: can we detect lipids in cells under resonance conditions?

Autori: Krzysztof Czamara, Adriana Adamczyk, Marta Stojak, Basseem Radwan, Malgorzata Baranska
Pubblicato in: Cellular and Molecular Life Sciences, 2020, ISSN 1420-682X
Editore: Birkhauser Verlag
DOI: 10.1007/s00018-020-03718-1

New Highly Sensitive and Specific Raman Probe for Live Cell Imaging of Mitochondrial Function

Autori: Pieczara, Anna Arellano Reyes, Ruben Arturo Keyes, Tia E. Dawiec, Patrycja Baranska, Malgorzata
Pubblicato in: ACS Sensors, Numero 9, 2024, ISSN 2379-3694
Editore: American Chemical Society
DOI: 10.1021/acssensors.3c02576

BODIPY‐Perylene Charge Transfer Compounds; Sensitizers for Triplet‐Triplet Annihilation Up‐conversion

Autori: Ruben Arturo Arellano‐Reyes; Amrutha Prabhakaran; Rengel Cane E. Sia; Julien Guthmuller; Keshav Kumar Jha; Tingxiang Yang; Benjamin Dietzek‐Ivanšić; Vickie McKee; Tia E. Keyes
Pubblicato in: Chemistry – A European Journal, Numero 29, 2023, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202300239

Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration

Autori: Haley, Ehlers; Arnaud, Nicolas; Frederik, Schavemaker; Jeroen P M, Heijmans; Martin, Bulst; Sebastiaan J, Trietsch; Lenie J, van den Broek
Pubblicato in: Front Immunol, Numero 14, 2023, ISSN 1664-3224
Editore: Frontiers Media S.A.
DOI: 10.3389/fimmu.2023.1118624

Triplet–Triplet Annihilation Upconversion by Polymeric Sensitizers

Autori: Keshav Kumar Jha Amrutha Prabhakaran Christopher S. Burke Marcus Schulze Ulrich S. Schubert Tia E. Keyes Michael Jäger Benjamin Dietzek Ivanšić
Pubblicato in: Journal of Physical Chemistry C, Numero 126, 2022, Pagina/e 4057–4066, ISSN 1932-7447
Editore: American Chemical Society
DOI: 10.1021/acs.jpcc.1c09897

Bifacial Dye Membranes: Ultrathin and Free-Standing although not Being Covalently Bound

Autori: Hupfer, M. L. Dellith, J. Seyring, M. Diegel, M. Dellith, A. Ghosh, S. Rettenmayr, M. Dietzek-Ivansic, B. Presselt, M.
Pubblicato in: Advanced Materials, 2023, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202204874

Evaluation of the passive permeability of antidepressants through pore-suspended lipid bilayer

Autori: Sarangi, Nirod Kumar Prabhakaran, Amrutha Roantree, Mark Keyes, Tia E.
Pubblicato in: Colloids and Surfaces B: Biointerfaces, 2024, ISSN 0927-7765
Editore: Elsevier BV
DOI: 10.1016/j.colsurfb.2023.113688

Non-peer reviewed articles (2)

Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip model

Autori: Pandian, Kanchana Huang, Luojiao Junaid, Abidemi Harms, Amy Van Zonneveld, Anton Jan Hankemeier, Thomas
Pubblicato in: BioRXiv, 2023, ISSN 2692-8205
Editore: bioRxiv
DOI: 10.1101/2023.12.03.569402

Triplet-triplet annihilation upconversion for calcium sensing

Autori: Andreeva, Valeriia D. Yang, Tingxiang Regeni, Irene Dietzek-Ivanšić, Benjamin Bonnet, Sylvestre A.
Pubblicato in: ChemRxiv, 2023, ISSN 2573-2293
Editore: ChemRxiv
DOI: 10.26434/chemrxiv-2023-7p1s5