Projektbeschreibung
Die Oogenese sichtbar werden lassen
Das EU-finanzierte Projekt JOLI hat die Oogenese zum Schwerpunkt. Diese streng regulierte Reifung und Produktion von Eizellen ist ein Schlüsselprozess der Entwicklung. Das Forschungsteam wird die Oogenese der Clytia hemisphaerica aus der Klasse der Hydrozoen, die optisch klar erkennbare Gonaden besitzt, mittels Echtzeit-Bildgebung beobachten. Die erstmalige Visualisierung des gesamten Prozesses von der Stammzelle bis zur Eizelle wird wichtige Erkenntnisse über den Übergang von der mitotischen Proliferation der Keimzellvorläufer zur Meiose liefern. Zudem wird JOLI die Funktion spezifischer Moleküle in der Oogenese sowie im Prozess der Meiose und Chromosomenpaarung untersuchen. Mehr Wissen über die Oogenese wird zur Ermittlung der Ursachen weiblicher Unfruchtbarkeit beitragen.
Ziel
Oocyte production is a key feature of animal development, comprising a series of carefully regulated events. This project will exploit a new experimental model, the hydrozoan Clytia hemisphaerica, to analyse by live imaging approaches the entire process of oogenesis in isolated female gonads from stem cell to oocyte for the first time. Analyses will focus on early events largely inaccessible in existing animal models, covering the transition from mitotic proliferation of germ cell precursors to meiotic entry. Key events include homologous chromosome pairing, synaptonemal complex formation, and meiotic double strand break formation. Clytia gonads are optically clear, simply organised and maintain oocyte production for several days ex-vivo. The three specific project objectives are 1) to define the spatiotemporal progression of early oogenesis through molecular cartography of the different precursor pools and cell morphology characterisation; 2) to establish conditions for long term imaging, including development of fluorescent markers; 3) to address the function of the key enzyme Spo11 by combining live imaging with CRISPR-mediated gene knockout, monitoring the movements of homologous chromosomes during pairing and identifying the sites of synaptonemal complex polymerisation. This multifaceted project represents a thematic shift for the applicant and involves a new collaboration between expert labs with complementary strengths in developmental, molecular, and cellular biology. Two-way transfer of knowledge will enable the candidate to develop new skills (microscopy, live imaging, transgenics) whilst introducing her existing expertise with cnidarian biology and bioinformatics to the host labs. The project will improve understanding of the cellular and molecular dynamics of meiosis, establish a new experimental model for oogenesis studies, and provide the candidate with a solid foundation of complementary experience to fulfill her long term career objectives.
Wissenschaftliches Gebiet
- natural sciencesearth and related environmental sciencesphysical geographycartography
- natural sciencesphysical sciencesopticsmicroscopy
- natural sciencesbiological sciencesgeneticsheredity
- natural sciencesbiological sciencesgeneticschromosomes
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenFinanzierungsplan
MSCA-IF-EF-RI - RI – Reintegration panelKoordinator
75005 Paris
Frankreich