Periodic Reporting for period 1 - MINIREX (Reconstitution of a Minimal Signaling-Active Plant Receptor Kinase Complex - Towards the Structural Determination of Ligand-Induced Activation Mechanisms)
Okres sprawozdawczy: 2021-01-01 do 2022-12-31
For plants, just as for all organisms, the first step of physiological response lies in the perception of environmental changes and adequate translation into biochemical events. To monitor and respond to alterations in extracellular conditions, plants deploy cell surface-localized receptors, such as receptor kinases (RKs).
The RK family represents one of the largest plant protein families and thereby poses a challenge to characterization via solely genetic approaches, even using state-of-the-art tools. Thus, despite the critical importance of understanding the fundamental basis of RK signalling, the characterization of a minimal functional RK complex remains elusive. I therefore combined both cell-based and cell-free approaches to address this critical knowledge gap.
During this project, two peer-reviewed open access publication have been published which were directly supported by the action.