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Targeting ASTROcytes with cutting-edge EDITING technologies to treat the Alexander disease

Descrizione del progetto

Un trattamento di editing genetico per la malattia di Alexander

La malattia di Alexander (AxD, Alexander Disease) è un disturbo neurodegenerativo autosomico dominante, indotto da mutazioni missenso nel gene che codifica per la proteina acida fibrillare gliale (GFAP, Glial Fibrillary Acidic Protein), una proteina chiave del filamento intermedio degli astrociti. Ciò porta alla formazione di aggregati GFAP e all’attivazione della risposta allo stress, compromettendo l’omeostasi del sistema nervoso centrale. Il progetto ASTRO-EDITING, finanziato dall’UE, mira a ripristinare la funzione degli astrociti correggendo gli hotspot mutazionali di GFAP. A tale scopo, gli scienziati seguiranno un approccio di editing genetico basato su vettori di virus adeno-associati. Il progetto fornirà la prova di concetto di editing in vivo negli astrociti come potenziale strategia di terapia genica per l’AxD, offrendo allo stesso tempo un nuovo approccio terapeutico per il trattamento di altri disturbi neurodegenerativi.

Obiettivo

Alexander disease (AxD) is an autosomal dominant neurodegenerative disorder caused by missense mutations in the gene encoding the glial fibrillary acidic protein (GFAP), the major intermediate filament protein in astrocytes. Accumulation of GFAP aggregates in Rosenthal fibres leads to impairment of proteasomal activity and hyperactivation of the stress response, thus compromising astrocyte functions and altering the homeostasis of the central nervous system (CNS). Currently, this orphan disease lacks a cure.
The proposed project aims at providing in vivo proof-of-concept of safety, efficiency and efficacy of a novel and definitive AAV gene therapy approach based on gene editing / base editing strategies targeting GFAP mutational hotspots to recover pathological phenotypes in astrocytes. The candidate expects to outline novel editing platforms for widespread in vivo targeting of CNS astrocytes that could be applied for the treatment of AxD, as proposed in ASTRO-EDITING, and prospectively for disease modelling studies and therapeutic treatments of other neurological and psychiatric disorders by targeting pathological pathways involved in primary astrocyte degeneration or dysfunctional/maladaptive astrogliosis.
The proposal addresses the European issue of Horizon 2020, in the social challenge “Health, Demographic Change and Wellbeing” endeavours for the call “Better health and care, economic growth and sustainable health disease”, subject SC1-BMC-04-2018 “Rare Disease European Joint Programme Cofund”.
The expertise of the host laboratory, the international environment of OSR and the personalized career development plan will significantly reinforce candidate's competences and knowledge in the gene therapy field, expand his portfolio of therapeutic areas, create a new research niche, and strengthen his organizational, management and interpersonal skills, thus favouring the progressive acquisition of independence as experienced researcher in the scientific community.

Campo scientifico (EuroSciVoc)

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.

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Coordinatore

OSPEDALE SAN RAFFAELE SRL
Contribution nette de l'UE
€ 183 473,28
Indirizzo
VIA OLGETTINA 60
20132 Milano
Italia

Mostra sulla mappa

Regione
Nord-Ovest Lombardia Milano
Tipo di attività
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Collegamenti
Costo totale
€ 183 473,28