This project encompassed a range of both scientific and non-scientific activities divided in 6 work packages (WP) the first three were delved to explore three scientific aims and the fourth to sixth to focus on dissemination and exploitation, training and project managing.
Work Package 1: Aim 1
This package aimed to investigate the methylation profiles of different cell types, focusing on senescence-related changes in healthy and leukemic cells. Despite encountering some challenges, such as limited sample sizes and variations, notable progress was made. Particularly, the study identified key methylation patterns related to a signaling pathway, shedding light on potential connections between aging, senescence, and leukemia. Further analysis of specific genes provided insights into their roles in cell regulation and potential implications for leukemia prognosis and treatment response.
Work Package 2: Aim 2
This package aimed to assess the impact of senolytic drugs on senescent cells in bone marrow, focusing on their effects on hematopoietic stem cells (HSCs) and leukemia cells. While facing challenges in sample acquisition and size, initial observations indicated a reduction in senescent cells following senolytic drug treatment compared to chemotherapy. Functional assays, including Colony Forming Unit (CFU) assays, were developed to evaluate stemness and differentiation capacity. These efforts were complemented by methylation studies aimed at identifying potential biomarkers for treatment response.
Work Package 3: Aim 3
Although this package was not pursued due to resource limitations and strategic decisions, it aimed to explore the methylation changes in senescent and non-senescent HSCs in leukemia mouse models and assess the efficacy of senolytic drugs in preventing disease progression.
Work Package 4: Dissemination and Exploitation:
This package focused on disseminating project findings through various channels, including 2 scientific publications, 1 Organisation of a Conference, 6 Social Media, 3 Mentions on Websites, 7 Participation to a Conference, 3 Participation to an Event other than a Conference or a Workshop and 3 activities were organised jointly with other EU project(s).
Work Package 5: Training:
This package aimed to enhance the researcher's skill set through training courses, scientific meetings, and workshops. I gained proficiency in various scientific techniques and bioinformatics tools, contributing to the successful execution of project tasks. Attendance at scientific meetings facilitated knowledge exchange and networking opportunities crucial for career development.
Work Package 6: Management:
This package focused on project management, ensuring effective coordination of scientific and financial aspects. Regular meetings with supervisors and project managers facilitated communication and decision-making, while collaborations with other institutions contributed to the organization of scientific events and adherence to project guidelines. Compliance with data management requirements demonstrated a commitment to transparent and accountable research practices.
