The major scientific and technical outcomes during the project duration and by the end of the project (M42):
- Synthesized and verified two multifunctional DNA oligo sequence (origami attachment, bioRE, fluorescent AgNC coordination) for the analytes AF-B1 and Ochratoxin A
- Synthesized NCs functionalized antibodies probes and oriented functionalization for complementary bioRE attachment sequence DNA strand conjugation
- Three DNA probes were designed and validated for N-gene of SARS-CoV-II as bio-RE
- Tri-peptide structure as bioRE was introduced as further bio-RE
- Binding rate analysis of the bio-RE with switchSENSE technology were performed
- Synthesized monodisperse AuNPs, oligo-functionalised for DNA origami attachment
- Optimized design, synthesis and purification parameters for DNA origami templates with different NP spacing (2-15 nm)
- Optimized DNA origami templates for array formation and surface integration on lipids
- Synthesized and characterised of DNA origami hybrids based on 3 of the available bio-RE types (DNA aptamer, antibody, DNA probe)
- Microtechnology processing of suitable immobilisation systems on Si and glass (SiO2/Parylene-F; SiO2/PMMA/APTES) with nanostructuring of binding point arrays for selective immobilization
- Optimized parameters for SiO2/Parylene-F and APTES based immobilization process
- Selective immobilized DNA origami hybrids structures (AFB1-Aptamer-Hybrid, Antibody-Hybrid, Probe A-Hybrid)
- Validation of aptamer based detection of AF-B1 by antibody-gold-nanoparticle conjugate
- Validation of the DNA origami hybrid sensor concept by benzophenone detection
The major achievements in terms of Exploitation and Innovation management by the end of the project (M42) are:
- Realization and updating (2 times) of an Exploitation plan and identification of 4 KER:
- Exploitation activities are underway with a spin-off from a partner institute that is interested in exploiting two of the KERs
- Further development of one KER to higher the TRL by serving evidence, illustration of technology and upscaling are in progress based on a national funded project in Germany
- New funding opportunities to further develop the DeDNAed sensor platform have been identified, in particular EIC Transition programme is considered to be the most relevant to the continuation of DeDNAed-generated biosensor
- The major output of IPR management is the recommendation of considering patenting or extension of existing patents to Europe and the USA (in progress)the generated IP as well as a considering licensing methods for the IPR
Activities undertaken to disseminate the results obtained during the project period up to the end of the project (M42):
- Defined and implemented comprehensive C&D strategy (PEDR) to involve different target groups
- Organized 2 dissemination workshops
- Coordinated participation in 36 conferences, 6 workshops, 1 pitch event, 5 other events
- Published 5 articles in peer reviewed, open access scientific journals
- Published 3 press releases and 11 non scientific articles
- Maintained consistent social media presence (LinkedIn, Youtube, Twitter)
- Construction and maintenance of the public website with Key facts of the project, consistently updated news and events and list of publications, posters and public deliverables and provision of link
