Skip to main content
Przejdź do strony domowej Komisji Europejskiej (odnośnik otworzy się w nowym oknie)
polski polski
CORDIS - Wyniki badań wspieranych przez UE
CORDIS
Zawartość zarchiwizowana w dniu 2024-04-30

Development of strategy and application of molecular tools to assess biodiversity in chicken genetic resources

Cel



The overall goal of the project is to experimentally evaluate strategic questions relating to the assessment of Biodiversity in the chicken using molecular information that can be measured in various ways at the DNA level. In the proposed project we will examine the level of point mutations between and within chicken populations. This will be relevant as a criterion to assess Biodiversity, and at the same time it will provide the background to adopt a completely new technology of DNA chips in poultry populations. Microsatellites are currently widely accepted as the most powerful tool to characterise Biodiversity at the molecular level. By collecting large samples from various breeds we will establish a complex genetic distance matrix to quantify Biodiversity in chickens based on microsatellites. Although theoretical studies have been carried out, questions related to the sampling strategy, number of markers and statistical analysis need still to be answered experimentally and will be approached in the proposed project. The project will deepen our understanding of Biodiversity not only in chickens, but also in comparative aspects by extending molecular studies of Biodiversity within Non-mammalian species. The major objectives addressed in the project are as follows:
1) To assess Biodiversity in a wide range of 50 chicken breeds using a set of 25 microsatellites and DNA pools of 50 individuals per breed for genotyping. This information will give insight in the currently existing Biodiversity in chickens, and by including commercial lines, to what extent they compare to unimproved local breeds and the Red Jungle fowl (Gallus gallus), the progenitor of all chickens. Gene mapping studies using microsatellites are already well established in the chicken. Some Participants of our project are far ahead in this technology to guarantee that the most appropriated markers in chickens will be used.
2) To investigate basic questions to estimate Biodiversity using microsatellites. Based on (1), 10 breeds representing a wide spectrum of the dendrogram will be selected for genotyping of 30 individuals per breed using 50 microsatellite loci. There have been theoretical studies providing estimates of the number of animals per breed to be sampled and number of microsatellites to be used. However, these predictions have not been tested experimentally in chickens to date. This approach will make possible a detailed analysis of the use of microsatellites, their size distribution and frequencies, and be of value to further diversity studies using this type of molecular marker.
3) To evaluate the level of point mutations in random non-coding DNA fragments for two reasons: a) to examine the feasibility of adopting DNA chip technology for the poultry populations and b) to use it as a criterion for the estimation of Biodiversity between and within chicken populations. To approach this question we will select 10 breeds and 10 individuals per breed, and we will sequence 10 random noncoding fragments per individual.
4) To develop a new statistical approach to assess Biodiversity. The statistical analysis of data to estimate genetic distances between populations and genetic variability within breeds is not straight forward and requires specific efforts. So far, no general consensus exists as to which of the many genetic distance estimates would be the best for analysis of within-species populations. Experimental data based on (1) (2) and (3) will provide the basis to determine the most appropriate method for estimating genetic distances.
5) To establish a chicken DNA collection and BIODIVERSITY database from the populations sampled for further research within the Community. A DNA collection of various breeds will extend the basis to search for further genetic polymorphisms identified in the Chicken gene mapping project. The BIODIVERSITY database will provide public access to the information collected and the analytical tools developed within the project.

Dziedzina nauki (EuroSciVoc)

Klasyfikacja projektów w serwisie CORDIS opiera się na wielojęzycznej taksonomii EuroSciVoc, obejmującej wszystkie dziedziny nauki, w oparciu o półautomatyczny proces bazujący na technikach przetwarzania języka naturalnego. Więcej informacji: Europejski Słownik Naukowy.

Aby użyć tej funkcji, musisz się zalogować lub zarejestrować

Program(-y)

Wieloletnie programy finansowania, które określają priorytety Unii Europejskiej w obszarach badań naukowych i innowacji.

Temat(-y)

Zaproszenia do składania wniosków dzielą się na tematy. Każdy temat określa wybrany obszar lub wybrane zagadnienie, których powinny dotyczyć wnioski składane przez wnioskodawców. Opis tematu obejmuje jego szczegółowy zakres i oczekiwane oddziaływanie finansowanego projektu.

Zaproszenie do składania wniosków

Procedura zapraszania wnioskodawców do składania wniosków projektowych w celu uzyskania finansowania ze środków Unii Europejskiej.

Brak dostępnych danych

System finansowania

Program finansowania (lub „rodzaj działania”) realizowany w ramach programu o wspólnych cechach. Określa zakres finansowania, stawkę zwrotu kosztów, szczegółowe kryteria oceny kwalifikowalności kosztów w celu ich finansowania oraz stosowanie uproszczonych form rozliczania kosztów, takich jak rozliczanie ryczałtowe.

CSC - Cost-sharing contracts

Koordynator

Institute for Animal Science and Animal Behaviour - Federal Agricultural Research Centre Braunschweig-Völkenrode
Wkład UE
Brak danych
Adres

31535 Neustadt am Rübenberge
Niemcy

Zobacz na mapie

Koszt całkowity

Ogół kosztów poniesionych przez organizację w związku z uczestnictwem w projekcie. Obejmuje koszty bezpośrednie i pośrednie. Kwota stanowi część całkowitego budżetu projektu.

Brak danych

Uczestnicy (6)

Moja broszura 0 0