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Zawartość zarchiwizowana w dniu 2024-04-30

Functional studies on the cytochrome-type nitrite reductase of thiosphaera pantotropha

Cel



As a key enzyme of the biological nitrogen cycle, bacterial nitrite reductase (cytochrome cd1) reduces nitrite but it also catalyses the reduction of oxygen to water. Cytochrome cd1 serves as an ideal model in elucidating three fundamental questions in biology:
- How do electrons move between haem groups?
- How is nitrite reduced to nitric oxide?
- How is oxygen reduced to water?
Based on the first crystal structure of cytochrome cd1, isolated from t soil bacterium T. pantotropha, the structural/ functional properties of this enzyme will be studied via various spectroscopic and electro- chemical methods.
Visible spectroscopy, especially stopped-flow experiments, will elucida the kinetics and catalytic mechanisms of this enzyme with respect to its nitrite and oxygen reducing capabilities. The effect of various substrates and inhibitors will be studied using the wild-type, specifically designed mutants and metal-substituted species.
In addition, redox-titrations and cyclovoltammetry will be used to stud the thermodynamics of inter- and intra electron-transfer processes between haem groups.

Dziedzina nauki (EuroSciVoc)

Program(-y)

Temat(-y)

Zaproszenie do składania wniosków

Brak dostępnych danych

System finansowania

RGI - Research grants (individual fellowships)

Koordynator

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
Wkład UE
Brak danych
Adres
SOUTH PARKS ROAD
OX1 3QU OXFORD
Zjednoczone Królestwo
Koszt całkowity
Brak danych

Uczestnicy (1)