Skip to main content
Przejdź do strony domowej Komisji Europejskiej (odnośnik otworzy się w nowym oknie)
polski pl
CORDIS - Wyniki badań wspieranych przez UE
CORDIS
Zawartość zarchiwizowana w dniu 2024-04-30

Kefa, a potassium efflux system in escherichia coli - molecular organisation and regulation of kefa locus, and structure characterization of kefa products

Cel



Regulation of potassium pool is essential for survival of bacteria submitted to hyperosmotic conditions. The potassium efflux systems KefA and KefD are believed to play a major role in regulating the potassium pool in Escherichia coli. The KefA system has been identified and cloned in the laboratory of Professor Booth in Aberdeen. The kefA locus has been sequenced and encodes three possible open reading frames. We will analyse the function of the three ORFs to determine which are responsible for KefA activity. The kefA products will be characterized in term of topology, cellular localization and activity. The regulation of ketA transcription and tlanslation will be studied by using gene fusion technology and primer extension. The influence of several factors on the activity of kefA, such as genetic background, medium osmolarity, growth phase and osmoprotectants presence will be investigated. The physiologic role of kefA will be studied by using defined kefA mutations created by reverse genetics.

Dziedzina nauki (EuroSciVoc)

Program(-y)

Temat(-y)

Zaproszenie do składania wniosków

Brak dostępnych danych

System finansowania

RGI - Research grants (individual fellowships)

Koordynator

UNIVERSITY OF ABERDEEN
Wkład UE
Brak danych
Adres
Institute of Medical Sciences, Foresthill
AB25 2ZD ABERDEEN
Zjednoczone Królestwo
Koszt całkowity
Brak danych

Uczestnicy (1)