Cel The Escherichia coli chaperonins GroEL and GroES facilitate protein folding in vivo and in vitro. However, the mechanism of action is not well understood. N-(5'Phosphoribosyl)anthranilate isomerase from E. coli (ePRAI), an alpha/Beta barrel protein, will be used as substrate for the chaperonin. The proposed project will address the following specific aspects of the activity of GroEL: 1) To determine if the chaperonin modifies the folding pathway of ePRAI. 2) To determine the role of ATP and GroES in the folding mechanism. 3) To determine at which stage of the folding pathway and what region of ePRAI interacts with GroEL. Dziedzina nauki nauki przyrodniczenauki biologicznebiochemiabiocząsteczkibiałkafałdowanie białek Program(-y) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Temat(-y) 0302 - Post-doctoral research training grants TL02 - Molecular Biology and Biochemistry Zaproszenie do składania wniosków Data not available System finansowania RGI - Research grants (individual fellowships) Koordynator University of Cambridge Adres Lensfield road CB2 1EW Cambridge Zjednoczone Królestwo Zobacz na mapie Wkład UE € 0,00 Uczestnicy (1) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Not available Hiszpania Wkład UE € 0,00 Adres Zobacz na mapie
