Cel
The Escherichia coli chaperonins GroEL and GroES facilitate protein folding in vivo and in vitro. However, the mechanism of action is not well understood. N-(5'Phosphoribosyl)anthranilate isomerase from E. coli (ePRAI), an alpha/Beta barrel protein, will be used as substrate for the chaperonin. The proposed project will address the following specific aspects of the activity of GroEL: 1) To determine if the chaperonin modifies the folding pathway of ePRAI. 2) To determine the role of ATP and GroES in the folding mechanism. 3) To determine at which stage of the folding pathway and what region of ePRAI interacts with GroEL.
Zaproszenie do składania wniosków
Data not availableSystem finansowania
RGI - Research grants (individual fellowships)Koordynator
University of Cambridge
Wkład UE
Brak danych
Adres
Lensfield Road
CB2 1EW Cambridge
Zjednoczone Królestwo
CB2 1EW Cambridge
Zjednoczone Królestwo
Koszt całkowity
Brak danych