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Zawartość zarchiwizowana w dniu 2024-05-14

Dynamics of the fusion pore in single-vesicle exocytosis

Cel



Although many of the proteins thought to be involved in regulated exocytosis have been cloned, their roles remain to be elucidated. I intend to study the function of specific proteins in the fusion of secretory vesicles with the plasma membrane in adrenal chromaffin cells. Using the techniques of membrane capacitance measurement and carbon-fiber amperometry (either individually or combined ) for detecting single vesicle fusion, I will characterize how the formation and evolution of the fusion pore -- the aqueous canal that connects the vesicle interiour with the extracellular medium -- depends on conditions such as calcium concentration, pH, temperature, and osmotic strength. I will also explore the effect of botulinum and tetanus neurotoxins.
After the basic characterization of the behaviour of the fusion pore, I will extend the studies to genetically modified cells that either lack the studied proteins or that express an abnormal form.

Dziedzina nauki (EuroSciVoc)

Program(-y)

Temat(-y)

Zaproszenie do składania wniosków

Brak dostępnych danych

System finansowania

RGI - Research grants (individual fellowships)

Koordynator

University of Edinburgh
Wkład UE
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Adres
Teviot Place
EH8 9AG Edinburgh
Zjednoczone Królestwo
Koszt całkowity
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Uczestnicy (1)