Analysis of ion channels in bacterial cells

Informacje na temat projektu

Identyfikator umowy o grant: FMBI983318

Projekt został zamknięty

Data rozpoczęcia 1 Lipca 1998

Data zakończenia 30 Czerwca 2000

Finansowanie w ramach

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Koszt całkowity Brak danych

Wkład UE Brak danych

Koordynowany przez

UNIVERSITY OF ABERDEEN
United Kingdom

Cel

Research objectives and content
The objective is to develop an understanding of the Kch(potassium channel) found in cells of the enteric bacterium Escherichia coli. Ion channels similar to Kch have major roles in the physiology of all eukaryotic cells, from simple yeasts to man. Over the last few years genes with the capacity to encode channels similar to those found in higher organisms have been identified in bacteria. The kch gene of E. coli encodes a protein that is similar in structure to the eukaryotic potassium channels. The major potassium transport systems of E coli are well described at both the genetic and physiological level and there is well-developed understanding of the regulation of potassium pools in this organism. This is, therefore, one of the best organisms in which to initiate the study of the role of potassium channels.
The channel protein is already under investigation in Professor Booth s laboratory and my work will complement those studies. Mutants will be produced in which the regulatory sites, identified by comparison with similar channels from higher organisms, will be altered. The effects of the mutations on channel activity will be investigated by patch clamp analysis of excised patches derived from bacterial protoplasts and by analysis of cell physiology to create an understanding of the role of the ion channel in the cell.
Training content (objective. benefit and expected impact)
Training in site-directed mutagenesis, analysis of cell physiology, controlled expression of membrane proteins in prokaryotic and eukaryotic cells, and electrophysiological analysis of channel activity will be gained. These advanced techniques will complement my basic training in molecular biology and cell physiology.
Links with industry / industrial relevance (22)
None for this project, but Prof. Booth's laboratory has good links with food and pharmaceutical companies to ensure rapid exploitation of any novel discoveries.

Dziedzina nauki (EuroSciVoc)

Klasyfikacja projektów w serwisie CORDIS opiera się na wielojęzycznej taksonomii EuroSciVoc, obejmującej wszystkie dziedziny nauki, w oparciu o półautomatyczny proces bazujący na technikach przetwarzania języka naturalnego.

• nauki przyrodnicze › nauki biologiczne › mikrobiologia › bakteriologia
• nauki przyrodnicze › nauki chemiczne › chemia nieorganiczna › litowce
• nauki przyrodnicze › nauki biologiczne › biochemia › biocząsteczki › białka
• medycyna i nauki o zdrowiu › medycyna kliniczna › fizjologia
• nauki przyrodnicze › nauki biologiczne › biologia molekularna

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Program(-y)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Temat(-y)

• 0302 - Post-doctoral research training grants
• TL02 - Molecular Biology and Biochemistry

Zaproszenie do składania wniosków

Data not available

System finansowania

RGI - Research grants (individual fellowships)

Koordynator

UNIVERSITY OF ABERDEEN

• Wkład UE: Brak danych
• Adres: Institute of Medical Sciences, Foresthill ; AB25 2ZD ABERDEEN ; Zjednoczone Królestwo
• Koszt całkowity: Brak danych