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Zawartość zarchiwizowana w dniu 2024-05-27

Catalytic mechanism of the aeromonas hydrophila metallo-beta-lactamase

Cel

Bacterial resistance to antibiotics is becoming an extremely worrying problem and beta-lactamase production is most often responsible for this phenomenon. In the frame of a general approach of the catalytic mechanisms of these enzymes, this project centres on the study of the Aeromonas hydrophila metallo-beta-lactamase. The residues which constitute the two putative zinc binding sites will be mutated to better understand their respective roles in the enzymatic properties of the protein. To do so, the modified enzymes will be produced, purified to protein homogeneity and their kinetic and (hopefully) structural characteristics determined. This should allow the design of potential inhibitors of this subclass of metallo-beta-lactamases. This program will involve extensive training in site-directed mutagenesis techniques, in protein chemistry enzyme kinetics and crystallization skills that will become more than ever precious in the post-genomic era.

Dziedzina nauki (EuroSciVoc)

Program(-y)

Temat(-y)

Brak dostępnych danych

Zaproszenie do składania wniosków

Brak dostępnych danych

System finansowania

RGI - Research grants (individual fellowships)

Koordynator

UNIVERSITE DE LIEGE
Wkład UE
Brak danych
Adres
Allée de la Chimie 3, Bètiment B6
4000 LIEGE
Belgia
Koszt całkowity
Brak danych