Descrizione del progetto
Un nuovo microscopio di raccolta cellulare
REVEAL è un progetto multidisciplinare finanziato dall’UE che intende sviluppare una tecnica di microscopia rivoluzionaria in grado di consentire l’immaginografia bidimensionale e tridimensionale di cellule vive. Utilizzando l’apprendimento automatico, il microscopio sarà in grado di percepire e interpretare eventi cellulari che possono provocare trasformazioni maligne. Fornirà agli utenti la possibilità di rilevare e prelevare vari tipi di cellule epatiche che saranno successivamente analizzate a livello molecolare e biochimico. Il progetto assocerà i fenotipi cellulari osservati al microscopio con i profili di espressione genica, generando conoscenze significative sull’origine cellulare del cancro al fegato.
Obiettivo
The REVEAL project aims at installing a novel microscope at the interface of instrumentation, machine learning and Biology. This new microscopy technique will rely heavily on neural networks to be able to perceive, interpret, conjecture, infer, anticipate and act. The present project intends to lay down the foundations of this ‘neuronal microscopy’ in the realms of 2D and 3D cell live imaging.
To achieve this goal, we will develop the necessary tools and the first instruments that will demonstrate the feasibility and the power of this new microscopy framework. In particular, we will develop a ‘cell picking neuronal microscope’ that will allow us to detect and collect different subtype of liver cells that are due to become tumoral and analyse them ahead of tumour manifestation. This analysis will help us to characterize the cellular heterogeneity known to exist at the origin of liver cancer. It will provide a direct mapping between cellular phenotypes observed under microscope and gene expression present in the early stages of liver cancer. Overall, we are thus defining a novel methodology, giving a central place to neuronal microscopy in the understanding of the cellular origin of the diseases.
REVEAL is a multidisciplinary project engaging 7 EU partners (2 being SMEs) from 4 countries covering the RTO, academic and industrial worlds, including medical doctors. An implementation plan is presented in the form of 6 work packages, 4 of them being technical. Synergy in communication and dissemination by the several partners and stakeholders will permit to maximize the project impact. Solutions to overcome the technological barriers, as well as appropriate deliverables, tasks, milestones and risks are presented, in order to complete the project objectives in due time . The REVEAL project outcomes are fully in line with the H2020 ICT-36-2020 call, subtopic iv, as it will develop novel methods and devices as research tools to understand the cellular origin of diseases.
Campo scientifico
Parole chiave
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Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
75015 PARIS 15
Francia