Obiettivo The existence of stable strains of prions, differing in incubation times, clinical features, and neuroanatomical PrPSc deposition, is disquieting. The structural and biological features which encode strains and determine their disease phenotype are obscure. Some strains, including BSE, easily traverse species barriers causing serious concerns in the EU. Promiscuous strains present a public health threat if they propagate through the food chain, while remaining either undetected or misidentified. These dangers are illustrated by the atypical cases of scrapie which have appeared throughout the EU, including in sheep hitherto considered genetically resistant, and by the identification of BSE in a goat in France. As yet there are no diagnostic procedures to identify TSE strains, except in some rare cases. We propose studying fundamental and applied aspects of prions strains and their relationship to the species barrier, and to use the knowledge generated to initiate diagnostic methodologies to detect strains and predict their epidemiological behaviour. We have assembled a powerful consortium of partners with proven experimental and industrial achievements. We will (i) systematically examine the properties of field strains and their species barriers when propagated (ii) examine strain-dependent biochemical and biophysical properties of PrPSc (iii) apply novel cryoelectron tomographical techniques to determine the 3-D structure (at 4 nm resolution) of PrPSc in various strains in neuronal (stem) cells (iv) study strain-specific features of prion infection in cultured cells, including their uptake receptors, (v) characterize strain dependent cytopathogenic mechanisms in infected cells. We will prepare novel diagnostic and research reagents such as a) possibly strain specific, PrP monoclonal antibodies, b) a goat PrP transgenic mouse, and c) novel ovine, caprine and bovine neuronal stem cells susceptible to prion infection. Campo scientifico medical and health scienceshealth sciencespublic healthepidemiologyepidemics preventionnatural sciencesbiological sciencescell biologymedical and health sciencesmedical biotechnologycells technologiesstem cellsengineering and technologyother engineering and technologiesfood technologyfood safetyagricultural sciencesanimal and dairy sciencedomestic animalsanimal husbandry Parole chiave atypical biological products prion species barrier strain Programma(i) FP6-FOOD - Food Quality and Safety: Thematic priority 5 under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) FOOD-2004-T5.4.5.3 - Investigating the fundamentals of TSE enabling the rational development of detection and control strategies Invito a presentare proposte FP6-2004-FOOD-3-B Vedi altri progetti per questo bando Meccanismo di finanziamento STREP - Specific Targeted Research Project Coordinatore THE HEBREW UNIVERSITY OF JERUSALEM Contributo UE Nessun dato Indirizzo The Authority for Research and Development, Edmond J. Safra Campus, Givat Ram JERUSALEM Israele Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (10) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto HET NEDERLANDS KANKER INSTITUUT Paesi Bassi Contributo UE Nessun dato Indirizzo Plesmanlaan 121 AMSTERDAM Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato KAROLINSKA INSTITUTET Svezia Contributo UE Nessun dato Indirizzo Nobels väg 5 STOCKHOLM Mostra sulla mappa Costo totale Nessun dato UNIVERSIDADE DE SANTIAGO DE COMPOSTELA Spagna Contributo UE Nessun dato Indirizzo Pazo de San Xerome- Praza do Obradoiro, s/n SANTIAGO DE COMPOSTELA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato INSTITUT PASTEUR Francia Contributo UE Nessun dato Indirizzo 25, rue du Docteur Roux PARIS Mostra sulla mappa Costo totale Nessun dato INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA Spagna Contributo UE Nessun dato Indirizzo Carretera de la Coruna, Km 7.4 MADRID Mostra sulla mappa Costo totale Nessun dato MABGENICS GMBH Germania Contributo UE Nessun dato Indirizzo 107 Frankfurter Strasse GIESSEN Mostra sulla mappa Costo totale Nessun dato INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE Francia Contributo UE Nessun dato Indirizzo 147 rue de l'Universite PARIS Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato NATIONAL VETERINARY INSTITUTE (SVA) Svezia Contributo UE Nessun dato Indirizzo UPPSALA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato THE VETERINARY LABORATORIES AGENCY OF NEW HAW, ADDLESTONE, SURREY, KT15 3NB Regno Unito Contributo UE Nessun dato Indirizzo 17 Smiths Square LONDON Mostra sulla mappa Costo totale Nessun dato HEINRICH HEINE UNIVERSITY OF DUESSELDORF Germania Contributo UE Nessun dato Indirizzo Moorenstrasse 5 DUESSELDORF Mostra sulla mappa Costo totale Nessun dato