Cel Neural stem cells (NSCs) can propagate in vitro while retaining ability to differentiate into neurons, astrocytes and oligodendrocytes. However, because of the nature of their developmental progression, NSCs isolated at different developmental stages or regions exhibit remarkable differences in their ability to yield specific neuron types. Specifically, NSCs grown in vitro rapidly lose access to the full neuronal spectrum. Limited NSC potential is one of the major impediments for applications in regenerative medicine, specifically for Parkinson’s and motoneuron-related diseases. Therefore, there is an enormous need to understand how NSCs self-renew.Human embryonic stem cells (hESCs) are known to provide access to early neural fates. We have recently isolated a novel type of early NSCs derived from hESCs termed rosette-NSCs (R-NSCs), which respond to such regional patterning cues. We proved the unique NSC stage of R-NSCs based on their cytoarchitecture, marker expression, stem cell properties and differentiation potential. Nevertheless, we only partially identified growth requirements and signaling pathways governing the R-NSCs stage.Here we would like to address these limitations by defining heterogeneity within R-NSCs and develop genetic strategies to prospectively isolate fully patternable R-NSCs. Generating transgenic hESC reporter lines will serve as reliable readout for defining R-NSC stage, identity and function, and will be used for establishing correlative genome-wide chromatin state and promoter methylation maps. Finally we will systematically probe function of extrinsic/intrinsic factors affecting R-NSC identity, neural patterning potential and epigenetic state. This should provide fundamental insights into the genetic/epigenetic mechanisms of neural patterning and ultimately result in novel conditions for the continued in vitro expansion of fully patternable R-NSC - a key step towards establishing a stable expandable universal NSC population. Dziedzina nauki medical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesbasic medicineneurologyparkinsonnatural sciencesbiological sciencesgeneticsepigenetics Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2009-RG - Marie Curie Action: "Reintegration Grants" Zaproszenie do składania wniosków FP7-PEOPLE-2010-RG Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IRG - International Re-integration Grants (IRG) Koordynator TEL AVIV UNIVERSITY Wkład UE € 100 000,00 Adres RAMAT AVIV 69978 Tel Aviv Izrael Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Lea Pais (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
