Final Report Summary - REM NETWORKS (Exploring the interface between cell metabolism and gene regulation: from mRNA interactomes to "REM Networks")
Furthermore, we developed a technology to systematically identify the RNA-binding domains (RBD) of RBPs in vivo (‘RBDmap’). Our studies uncovered nearly 1,200 RBDs from more than 500 RBPs. This information has implications for structural aspects of RNA-protein interactions, post-transcriptional gene regulation, and metabolism. It also serves to instruct mutagenesis experiments to determine the physiological functions of RNA-binding by classical metabolic enzymes and other RBPs. Overall, the project has yielded numerous unexpected, profound insights into RNA biology in general and RBPs specifically.