Activity-Based Profiling of Glycoprocessing Enzymes for Human Health and a Sustainable Society

Informazioni relative al progetto

CARBOCENTRE

ID dell’accordo di sovvenzione: 951231

DOI 10.3030/951231

Data della firma CE 18 Dicembre 2020

Data di avvio 1 Giugno 2021

Data di completamento 31 Maggio 2027

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale € 9 057 250,00

Contributo UE € 9 057 250,00

Coordinato da

UNIVERSITEIT LEIDEN
Netherlands

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (26)

Control of Substrate Conformation by Hydrogen Bonding in a Retaining β-Endoglycosidase

Autori: Alba Nin-Hill, Albert Ardevol, Xevi Biarnés, Antoni Planas, Carme Rovira
Pubblicato in: Chemistry—A European Journal, Numero 29, 2023, Pagina/e e202302555, ISSN 1521-3765
Editore: Wiley-VCH GmbH
DOI: 10.1002/chem.202302555

Reaction Mechanism of Glycoside Hydrolase Family 116 UtilizesPerpendicular Protonation

Autori: Salila Pengthaisong, Beatriz Piniello, Gideon J. Davies, Carme Rovira, and James R. Ketudat Cairns
Pubblicato in: ACS Catalysis, Numero 13, 9, 2023, Pagina/e 5850–5863, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.3c00620

Human O-GlcNAcase Uses a Preactivated Boat-skew Substrate Conformation for Catalysis. Evidence from X-ray Crystallography and QM/MM Metadynamics

Autori: Martín Calvelo, Alexandra Males, Matthew G. Alteen, Lianne I. Willems, David J. Vocadlo, Gideon J. Davies and Carme Rovira
Pubblicato in: ACS Catalysis, Numero 13, 20, 2023, Pagina/e 13151-13845, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.3c02378

Trans-cyclosulfamidate mannose-configured cyclitol allows isoform-dependent inhibition of GH47 α-d-mannosidases through a bump–hole strategy

Autori: Alexandra Males, Ken Kok, Alba Nin-Hill, Nicky de Koster, Sija van den Beukel, Thomas J. M. Beenakker, Gijsbert A. van der Marel, Jeroen D. C. Codée, Johannes M. F. G. Aerts, Herman S. Overkleeft, Carme Rovira, Gideon J. Davies, and Marta Artola
Pubblicato in: Chemical Science, Numero 14, 2023, Pagina/e 13581–13586, ISSN 1478-6524
Editore: Royal Society of Chemistry
DOI: 10.1039/d3sc05016e

An Orthogonally Protected Cyclitol for the Construction of Nigerose- and Dextran-Mimetic Cyclophellitols.

Autori: Tim P Ofman; Florian Küllmer; Gijsbert A. van der Marel; Jeroen D. C. Codée; Herman S. Overkleeft
Pubblicato in: Organic Letters, 23(24), 9516 - 9519, Numero 23 (24), 2021, Pagina/e 9516-9519, ISSN 1523-7060
Editore: American Chemical Society
DOI: 10.1021/acs.orglett.1c03723

Design and Synthesis of Exocyclic Cyclitol Aziridines as Potential Mechanism-Based Glycosidase Inactivators

Autori: Tim P. Ofman, Prof. Dr. Gijsbert A. van der Marel, Prof. Dr. Jeroen D. C. Codée, Prof. Dr. Hermen S. Overkleeft
Pubblicato in: European Journal of Organic Chemistry, Numero 26,16, 2023, Pagina/e e202300186, ISSN 1099-0690
Editore: Wiley-VCH GmbH
DOI: 10.1002/ejoc.202300186

Activity-based protein profiling reveals dynamic substrate-specific cellulase secretion by saprotrophic basidiomycetes

Autori: Nicholas G. S. McGregor; Casper de Boer; Mikhaaeel Santos; Mireille Haon; David Navarro; Sybrin Schroder; Jean-Guy Berrin; Herman S. Overkleeft; Gideon J. Davies
Pubblicato in: Biotechnology for Biofuels and Bioproducts, Numero 15, 2022, ISSN 2731-3654
Editore: BioMed Central Ltd.
DOI: 10.1186/s13068-022-02107-z

Molecular basis for bacterial N-glycosylation by a soluble HMW1C-like N-glycosyltransferase

Autori: Beatriz Piniello; Javier Macías-León; Shun Miyazaki; Ana García-García; Ismael Compañón; Mattia Ghirardello; Víctor Taleb; Billy Veloz; Francisco Corzana; Atsushi Miyagawa; Carme Rovira; Ramon Hurtado-Guerrero
Pubblicato in: Nature Communications, Numero 14, 2023, Pagina/e 5785, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-41238-1

Computer Simulation to Rationalize “Rational” Engineering of Glycoside Hydrolases and Glycosyltransferases

Autori: Joan Coines, Irene Cuxart, David Teze, and Carme Rovira
Pubblicato in: The Journal of Physical Chemistry B, Numero 126 (4), 2022, Pagina/e 802–812, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.1c09536

Rational tuning of the reactivity of three-memberet heterocycle ring openings via SN2 reactions

Autori: T. Hansen, A. Nin-Hill, J. D. Codée, T. A. Hamlin, C. Rovira
Pubblicato in: Chemistry European Journal, Numero 28, 2022, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202201649

Conformational and Electronic Variations in 1,2- and 1,5a-Cyclophellitols and their Impact on Retaining α-Glucosidase Inhibition

Autori: Tim P. Ofman, Jurriaan J. A. Heming, Alba Nin-Hill, Florian Küllmer, Elisha Moran, Megan Bennett, Roy Steneker, Anne-Mei Klein, Gijs Ruijgrok, Ken Kok, Zach W. B. Armstrong, Johannes M. F. G. Aerts, Gijsbert A. Marel, Carme Rovira, Gideon J. Davies, Marta Artola, Jeroen D. C. Codée, Herman S. Overkleeft
Pubblicato in: Chemistry—A European Journal, Numero 30, 2024, Pagina/e e202400723, ISSN 1521-3765
Editore: Wiley-VCH GmbH
DOI: 10.1002/chem.202400723

A multiplexing activity-based protein profiling platform for dissection of a native bacterial xyloglucan-degrading system

Autori: Nicholas McGregor ,Casper de Boer ,Quentin Foucart ,Thomas Beenakker ,Wendy Offen ,Jeroen Codee ,Lianne Willems ,Herman Overkleeft ,Gideon Davies
Pubblicato in: ACS Central Science, Numero 9, 12, 2023, Pagina/e 2306–2314, ISSN 2374-7943
Editore: American Chemical Society
DOI: 10.1021/acscentsci.3c00831

Mechanism-based heparanase inhibitors reduce cancer metastasis in vivo

Autori: Casper de Boer; Zachary Armstrong; Vincent A. J. Lit; Uri Barash; Gijs Ruijgrok; Ilanit Boyango; Merle M. Weitzenberg; Sybrin P. Schröder; Alexi J. C. Sarris; Nico J. Meeuwenoord; Pedro Bule; Yasmine Kayal; Neta Ilan; Jeroen D. C. Codée; Israel Vlodavsky; Herman S. Overkleeft; Gideon J. Davies; Liang Wu
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 119, 31, 2022, Pagina/e e2203167119, ISSN 1091-6490
Editore: National Academy of Sciences
DOI: 10.1073/pnas.2203167119

Enzymatic Hydrolysis of Human Milk Oligosaccharides. The Molecular Mechanism of Bifidobacterium Bifidum Lacto-N-biosidase

Autori: Irene Cuxart, Joan Coines, Oriol Esquivias, Magda Faijes, Antoni Planas, Xevi Biarnés, and Carme Rovira
Pubblicato in: ACS Catalysis, Numero 12,8, 2022, Pagina/e 4737-4743
Editore: American Chemical Society
DOI: 10.1021/acscatal.2c00309

Synthesis of broad-specificity activity-based probes for exo-beta-mannosidases

Autori: Nicholas G. S. McGregor; Chi-Lin Kuo; Thomas J. M. Beenakker; Chun-Sing Wong; Wendy A. Offen; Zachary Armstrong; Bogdan I. Florea; Jeroen D. C. Codée; Herman S. Overkleeft; Johannes M. F. G. Aerts; Gideon J. Davies
Pubblicato in: Organic & Biomolecular Chemistry, Numero 20, 2022, Pagina/e 877-886, ISSN 1477-0520
Editore: Royal Society of Chemistry
DOI: 10.1039/d1ob02287c

Molecular Basis for Inhibition of Heparanases and β‑Glucuronidasesby Siastatin B

Autori: Yurong Chen, Adrianus M. C. H. van den Nieuwendijk, Liang Wu, Elisha Moran, Foteini Skoulikopoulou, Vera van Riet, Hermen S. Overkleeft, Gideon J. Davies and Zachary Armstrong
Pubblicato in: Journal of the American Chemical Society, Numero 146, 1, 2024, Pagina/e 125-133, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.3c04162

Combinatorial assembly and design of enzymes

Autori: R. Lipsh-Sokolik, O. Khersonsky, S. P. Schröder, C. de Boer, S.-Y. Hoch, G. J. Davies, H. S. Overkleeft and S. J. Fleishman
Pubblicato in: Science, Numero 379, 6628, 2023, Pagina/e 195-201, ISSN 1095-9203
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.ade9434

The development of a broad-spectrum retaining β-exo-galactosidase activity-based probe

Autori: Kuo, C.L.; Su, Q.; Nieuwendijk, A.M.C.H. van den; Beenakker, T.J.M.; Offen, W.A.; Willems, L.I.; Boot, R.G.; Sarris, A.J.C.; Rosa Alcalde Marques, A.R.A.; Codée, J.D.C.; Marel, G.A. van der; Florea, B.I.; Davies, G.J.; Overkleeft, H.S.; Aerts, J.M.F.G.
Pubblicato in: Organic & Biomolecular Chemistry, Numero 21, 2023, Pagina/e 7813-7820, ISSN 1477-0520
Editore: Royal Society of Chemistry
DOI: 10.1039/d3ob01261a

Xylose-Configured Cyclophellitols as Selective Inhibitors for Glucocerebrosidase

Autori: Qin Su; Sybrin P. Schröder; Lindsey T. Lelieveld; Maria J. Ferraz; Marri Verhoek; Rolf G. Boot; Herman S. Overkleeft; Johannes M. F. G. Aerts; Marta Artola; Chi-Lin Kuo
Pubblicato in: Chembiochem, Numero 22 (21), 2021, Pagina/e 3090-3098, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202100396

Evolution of Phosphorylase Activity in an Ancestral Glycosyltransferase

Autori: Jorick Franceus, José Pablo Rivas-Fernández, Jolien Lormans, Carme Rovira and Tom Desmet
Pubblicato in: ACS Catalysis, Numero 14, 5, 2024, Pagina/e 3103−3114, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.3c05819

β-l- Arabino furano-cyclitol Aziridines Are Covalent Broad-Spectrum Inhibitors and Activity-Based Probes for Retaining β-l-Arabinofuranosidases

Autori: Borlandelli V, Offen W, Moroz O, Nin-Hill A, McGregor N, Binkhorst L, Ishiwata A, Armstrong Z, Artola M, Rovira C, Davies GJ, Overkleeft HS.
Pubblicato in: ACS Chemical Biology, Numero 18 (12), 2023, Pagina/e 2564–2573, ISSN 1554-8937
Editore: American Chemical Society
DOI: 10.1021/acschembio.3c00558

Fluorescence polarisation activity-based protein profiling for the identification of deoxynojirimycin-type inhibitors selective for lysosomal retaining alpha- and beta-glucosidases

Autori: Daniël van der Gracht; Rhianna J. Rowland; Véronique Roig-Zamboni; Maria J. Ferraz; Max Louwerse; Paul P. Geurink; Johannes M. F. G. Aerts; Gerlind Sulzenbacher; Gideon J. Davies; Herman S. Overkleeft; Marta Artola
Pubblicato in: Chemical Science, Numero 14, 2023, Pagina/e 9136–9144, ISSN 2041-6539
Editore: Royal Society of Chemistry
DOI: 10.1039/d3sc01021j

In silico modelling of the function of disease-related CAZymes

Autori: Alba Nin-Hill, Beatriz Piniello, Carme Rovira
Pubblicato in: Essays in Biochemistry, Numero 67 (3), 2023, Pagina/e 355–372, ISSN 0071-1365
Editore: Portland Press, Ltd.
DOI: 10.1042/ebc20220218

1,6-epi-Cyclophellitol cyclosulfamidate is a bona fide lysosomal α-glucosidase stabilizer for the treatment of Pompe disease

Autori: Ken Kok; Chi-Lin Kuo; Rebecca E. Katzy; Lindsey T. Lelieveld; Liang Wu; Véronique Roig-Zamboni; Gijsbert A. van der Marel; Jeroen D. C. Codée; Gerlind Sulzenbacher; Gideon J. Davies; Herman S. Overkleeft; Johannes M. F. G. Aerts; Marta Artola
Pubblicato in: Journal of the American Chemical Society, Numero 144, 2022, Pagina/e 14819-14827, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.2c05666

4-O-Substituted Glucuronic Cyclophellitols are Selective Mechanism-Based Heparanase Inhibitors

Autori: V. Borlandelli, Z. Armstrong, A. Nin-Hill, J. Codée, Ll. Raich, M. Artola, C. Rovira, G. Davies, H. S. Overkleeft
Pubblicato in: ChemMedChem, 2022, ISSN 1860-7179
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.202200580

Glycosidase mechanisms: Sugar conformations and reactivity in endo- and exo-acting enzymes

Autori: Mariana Abrahão Bueno Morais, Alba Nin-Hill, Carme Rovira
Pubblicato in: Current Opinion in Chemical Biology, Numero 74, 2023, Pagina/e 102282, ISSN 1879-0402
Editore: Elsevier B.V.
DOI: 10.1016/j.cbpa.2023.102282

Diritti di proprietà intellettuale

Patent (2)

PSEUDO-DISACCHARIDE COMPOUNDS

Numero candidatura/pubblicazione: 20 23050231
Data: 2023-04-28
Candidato/i: UNIVERSITY OF YORK

PSEUDO-DISACCHARIDE COMPOUNDS

Numero candidatura/pubblicazione: 20 23050231
Data: 2023-04-28
Candidato/i: UNIVERSITEIT LEIDEN