Cel The objective of this proposal is to probe in aqueous solution protein complexes which are both heterogeneous and possess highly variable stoichiometries. The study of heterogeneous protein systems by conventional means is very challenging since most current biophysical methods perform best for pure solutions of isolated components - yet proteins exert in the majority of cases their biological functionality through forming complexes. We propose in this application that the key to study such systems is to operate in much smaller volumes than in conventional biophysical experiments. We will use microfluidics to obtain information about the physical properties of protein complexes in real time through quantitative micron-scale measurements of mass transport of molecular species under the action of diffusion and electric or centrifugal fields. Furthermore, by working in small volumes, we will study nucleation phenomena inherent to many protein self-assembly phenomena on the level of single nucleation events by segregating individual nuclei into spatially distinct compartments. Modern microfabrication techniques that allow for the manipulation of liquids on the picolitre scales required for this project are available and will be exploited, but the potential of this technology to define experimentally highly heterogeneous protein complexes in terms of their key fundamental physical properties, such as the hydrodynamic radius, charge and mass, and shed light on the physical basis of protein self-assembly, have remained unexploited. Using this approach, we will explore biological problems of fundamental and practical importance characterised by heterogeneity, including functional chaperone complexes, formation and detection of amyloid oligomers and studies of complex biomolecular mixtures. This programme will deliver fundamentally new approaches to study heterogeneous protein complexes and will shed light on the physical principles that govern protein self-assembly. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesbiophysics Program(-y) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) ERC-SG-PE3 - ERC Starting Grant - Condensed matter physics Zaproszenie do składania wniosków ERC-2013-StG Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-SG - ERC Starting Grant Instytucja przyjmująca THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Wkład UE € 1 499 895,00 Adres TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Zjednoczone Królestwo Zobacz na mapie Region East of England East Anglia Cambridgeshire CC Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Renata Schaeffer (Ms.) Kierownik naukowy Tuomas Pertti Jonathan Knowles (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych Beneficjenci (1) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Zjednoczone Królestwo Wkład UE € 1 499 895,00 Adres TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Zobacz na mapie Region East of England East Anglia Cambridgeshire CC Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Renata Schaeffer (Ms.) Kierownik naukowy Tuomas Pertti Jonathan Knowles (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
