Cel The neuronal membrane is the target of specific molecular interactions involved in the development of Alzheimer’s disease (AD). The 2 hallmarks of the disease, extracellular amyloid β plaques and intracellular Tau neurofibrillatory tangles, are inherently linked to the membrane, as this biointerface induces and influences amyloid fibrillation. Moreover amyloid peptides selectively interact with the membrane depending of its composition, leading to toxicity mechanisms that are still poorly understood at the molecular scale. This project aims at providing a better understanding of the toxicity mechanisms of the amyloid peptides Aβ1-42 and Tau involved in AD on the neuronal membrane. Using Atomic Force Microscopy (AFM) with Carbon NanoTube (CNT) probes, we will obtain high-resolution imaging of these interactions at the nanometer scale. A unique functionalization of CNT tips with amyloid peptides will also allow true single molecule force spectroscopy of peptide/lipid interactions, unraveling their driving forces. With a progressive increase of the complexity in our system, we will, for the first time, investigate a potential synergy between Aβ1-42 and Tau and will develop an innovative approach to study live neurons by AFM imaging and force spectroscopy. Our study will establish new powerful methods to study the interactions of peptides with membranes or cells at the nanometer scale. We will not only contribute to the understanding of the deleterious effect of amyloid peptides on the neuronal membrane interface but also to the design of efficient preventive or therapeutic treatments, still lacking today. Dziedzina nauki medical and health sciencesbasic medicineneurologydementiaalzheimernatural sciencesphysical sciencesopticsmicroscopynatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural sciencesphysical sciencesopticsspectroscopy Słowa kluczowe Alzheimer’s disease Atomic Force Microscopy Carbon Nanotubes Functionalization Biointerface Lipid Membrane Amyloid peptides Program(-y) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Temat(-y) MSCA-IF-2017 - Individual Fellowships Zaproszenie do składania wniosków H2020-MSCA-IF-2017 Zobacz inne projekty w ramach tego zaproszenia System finansowania MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Koordynator UNIVERSITE DE BORDEAUX Wkład UE netto € 173 076,00 Adres PLACE PEY BERLAND 35 33000 Bordeaux Francja Zobacz na mapie Region Nouvelle-Aquitaine Aquitaine Gironde Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 173 076,00
