Opis projektu
Trójwymiarowy model in vitro demencji
U pacjentów cierpiących na otępienie czołowo-skroniowe obserwuje się degenerację płatów czołowych i skroniowych mózgu związaną z powstawaniem agregatów białkowych. Ze względu na obecny brak terapii otępienia czołowo-skroniowego, naukowcy z finansowanego przez UE projektu FTD-Organoids wykorzystają trójwymiarowe kultury organoidów mózgu wyhodowanych z ludzkich zarodkowych komórek macierzystych do zbudowania pierwszego modelu mózgu in vitro choroby. Naukowcy scharakteryzują ten model na poziomie komórkowym i molekularnym oraz zbadają mechanizmy leżące u podstaw patofizjologii otępienia czołowo-skroniowego. Wyniki projektu mogą pomóc w określeniu nowatorskich celów choroby na potrzeby opracowania nowych form leczenia.
Cel
Frontotemporal dementia (FTD) is the second most common form of dementia. A pathological hallmark is the formation of protein aggregates that consist mainly of Tau or TDP43. Patients suffer from the degeneration of the frontal and temporal lobes with no effective treatments being available. The recent advancement of 3D brain organoid cultures grown from embryonic stem cells offer new possibilities to study disease mechanisms of neurological disorders and help validate therapeutic interventions. I therefore propose to generate the first cerebral organoid models of FTD. The new models will first be thoroughly characterized by immunohistochemical stainings and biochemical analysis. Furthermore, I will determine neuroaxonal degeneration, visualize protein aggregates and their cell-to-cell spreading and analyze the nucleation of the pathological protein conformation. In addition, I will determine if FTD-organoids recapitulate the brain region- and cell type-specific vulnerability that are observed in patients. FTD has also been linked to aberrant phase transition of proteins that can lead to disturbances in proteome and RNA homeostasis. However, molecular mechanisms and consequences of this processes in disease conditions remain largely unknown. I therefore propose to study the dynamics and molecular composition of stress granules, which are functional biomolecular condensates that arise through liquid-liquid phase separation during stress conditions, in FTD cerebral organoids. Disease-associated changes in stress granule dynamics and thus disturbances in RNA metabolism and regulation of neuronal translational might result in the lack of essential neuronal proteins. I will therefore identify and quantify key mRNA and protein components of neuronal stress granules in brain organoids. In summary, this project will provide new mechanistic insights into disease mechanisms of FTD and open new possibilities of therapeutic interventions.
Dziedzina nauki
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomics
- medical and health sciencesmedical biotechnologycells technologiesstem cells
- medical and health sciencesbasic medicineneurologydementia
- natural sciencesbiological sciencesgeneticsRNA
- medical and health sciencesbasic medicinephysiologyhomeostasis
Słowa kluczowe
Program(-y)
Temat(-y)
System finansowania
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordynator
1030 Wien
Austria