Cel Technologies for T cell visualisation and T cell depletion/enrichment are steadily making their way into the clinic and developments seem dictated by availability and reliability of the peptide-Major Histocompatibility Complex (pMHC) reagents used. A bottleneck has always been the laborious process of expression, refolding and purification of individual pMHCs. Recently, conditional MHC technology, pioneered in the host laboratory, solved this problem in part by enabling high-throughput loading of one MHC allele with a wide panel of peptide ligands. However, the rapid, parallel generation of a variety of MHC alleles is still a major hurdle. Therefore, proposed research aims at the total chemical synthesis of conditional MHC platforms, to make high-throughput generation of different MHC alleles reality. Recent advances in solid phase peptide synthesis (SPPS) have now enabled the generation of long polypeptides and even small proteins. This proposal aims to use SPPS rather than biological expression systems for total protein synthesis. The major advantage of using chemical techniques for protein synthesis is (1) that it allows the facile introduction of (non-natural) amino acids with functionalities that stabilise the protein by enhanced H-bonding, intrastrand crosslinking or intramolecular cross-linking (cyclisation) and that enable immobilisation or conjugation, (2) that protein yields are higher, and (3) that it simplifies the controlled generation of GMP grade proteins for biomedical use.The outcomes of this project will benefit the development of adoptive T cell therapy and will enable epitope scans through entire genomes of emerging pathogens (e.g. pandemic H1N1 Influenza), thus contributing to vaccine development. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health scienceshealth sciencesinfectious diseasesRNA virusesinfluenzanatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesgeneticsgenomesmedical and health sciencesmedical biotechnologycells technologies Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2009-IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Zaproszenie do składania wniosków FP7-PEOPLE-2009-IEF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IEF - Intra-European Fellowships (IEF) Koordynator STICHTING HET NEDERLANDS KANKER INSTITUUT-ANTONI VAN LEEUWENHOEK ZIEKENHUIS Wkład UE € 161 748,80 Adres PLESMANLAAN 121 1066 CX Amsterdam Niderlandy Zobacz na mapie Region West-Nederland Noord-Holland Groot-Amsterdam Rodzaj działalności Research Organisations Kontakt administracyjny Henri Van Luenen (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych