Cel Phenotypic heterogeneity – physical diversity amongst genetically identical individuals exposed to the same environmental conditions – is a phenomenon that has caught the interest of biologists for decades. Although recent technological advances have revolutionised our understanding of the mechanistic bases of phenotypic heterogeneity, its adaptive significance and associated biological function(s) remain largely obscure. Two hypotheses have been proffered: (i) the “bet hedging” hypothesis, which postulates that phenotypic heterogeneity is a risk-spreading strategy facilitating population survival in uncertain environments, and (ii) the “division of labour” hypothesis, which proposes that phenotypic heterogeneity can increase population efficiency by partitioning labour (or resources). However, despite great interest in this area, empirical evidence for the biological significance of phenotypic heterogeneity is severely lacking. Here, I propose a series of experiments that combine molecular genetics, state of the art single cell analysis techniques and metabolomics to directly test the biological significance of a recently characterised example of phenotypic heterogeneity: ON/OFF colanic acid capsule expression in the bacterial model system Pseudomonas fluorescens SBW25. By providing a direct, experimental test of hitherto theoretical hypotheses, the proposed experiments have the potential to provide some of the first empirical insight into why phenotypic heterogeneity is such a widespread phenomenon. In addition to being of academic interest, our results will have direct relevance for industries involved in the management of infectious microbes (public health, veterinary science and agriculture) and the use of microbes in product manufacture (biotechnology) and toxin breakdown (bioremediation). Dziedzina nauki natural sciencesbiological sciencesgeneticsmedical and health scienceshealth sciencespublic healthnatural sciencesbiological sciencesmolecular biologymolecular geneticsagricultural sciencesveterinary sciencesengineering and technologyenvironmental biotechnologybioremediation Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2012-IIF - Marie Curie Action: "International Incoming Fellowships" Zaproszenie do składania wniosków FP7-PEOPLE-2012-IIF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IIF - International Incoming Fellowships (IIF) Koordynator EIDGENOESSISCHE ANSTALT FUER WASSERVERSORGUNG ABWASSERREINIGUNG UND GEWAESSERSCHUTZ Wkład UE € 192 622,20 Adres UBERLANDSTRASSE 133 8600 Dubendorf Szwajcaria Zobacz na mapie Region Schweiz/Suisse/Svizzera Zürich Zürich Rodzaj działalności Other Kontakt administracyjny Laura Klein (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
